Purification & Analysis of Bispecific Antibodies: Cut three steps into two!

Imagine a downstream chromatography process for a bispecific antibody that is faster and cheaper than your current one, and suits for platform use – appealing, isn’t it? We thought so and set out to see what happens if we replace the two intermediate affinity steps by a mixed mode followed by HIC polishing. Process control was performed using HPLC in SEC and CEX mode. The results are stunning – please be invited to see the details!

 More information on the used columns and media in the above application note can be obtained from SerCoLab.